Abstract
In order to design an optimum liposome immunoadjuvant for tumor vaccines, we investigated the relationship between liposomal surface charge and adjuvant action. Multilamellar vesicles (MLV) showed substantial increase in both cell-association and uptake by macrophages following enhancement of the degree of liposomal positive charge. Consistent with this, MLVs containing soluble ovalbumin (OVA) functioned increasingly as a more potent inducer of antigen-specific cytotoxic T lymphocyte (CTL) responses and antibody production after increasing the degree of liposomal positive charge. Furthermore, we examined the in vivo anti-tumor effect of various charged liposomal antigens using OVA and OVA-expressing transfectant, EG7. Vaccinal effects of MLV containing OVA were found to be dependent on the degree of liposomal positive charge. These results indicate that the positive charge on the surface of liposomes represents an important factor in enhancing their immunoadjuvancy in the induction of antigen-specific immune responses and vaccinal effects against tumors.
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