有機酸及び塩基による貝毒吸着精製の試み

Abstract

It is attempted to purify “Venerupin”, a poison found in shortneck clam and oyster in Lake Hamana, by using benzoic acid or quinine as an adsorbent. The attempt proved unsuccessful, but afforded new examples of loss of the poison and formation of foreign poison by a chemical treatment. When benzoic acid was precipitated by adding concentrated hydrochloric acid into a crude “Venerupin” solution containing sodium benzoate, the poison was found only in the supernatant liquid, and loss of the poison and formation of muscarine-like poison were observed (Table 1). On the other hand, when benzoic acid was precipitated by adding saturated solution of benzoic acid in 99% ethanol into the poison solution, the original poison was found without loss in the supernatant liquid, and the toxicity was slightly increased (Table 2). Formation of muscarinelike poison was not observed. Quinine treatment also caused loss of the poison (Table 3) but not formation of muscarinelike poison.