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Abstract
In this study, antimicrobial effect of mixed ultra-fine bubble liquids on two types of bacteria; spore-forming bacteria (Bacillus subtilis var. natto) and the environmental bacteria was investigated. We used ultrapure water (UPW), mixed ultra-fine bubble water (UFB), mixed ultra-fine ozone-rich bubble water (UFORB), and water containing ozone (OZ). Viable bacteria count (VBC) of UFB agreed with that of UPW within the experimental errors (p < 0.05). Low VBC (High effect) of UFORB and OW exhibited on B. subtilis var. natto. Moreover, complete sterilization of UFORB was obtained on the environmental bacteria. For discussing the experimental results, we observed number density against particle diameter, effect of dilution and elapsed time after stopped the ultra-fine bubble generator. Difference between UFB and UFORB exhibited in case of distribution of particle diameter (peak diameter and averaged diameter). The bactericidal effect on B. subtilis var. natto persisted after 360 min (6 h), but disappeared after 1440 min (24 h). On the other hand, the bactericidal effect against the environmental bacteria was high even after 1440 min (24 h).
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