へん平上皮由来しゅようにおける細胞骨格成分の免疫組織化学的研究

Abstract

Cytoskeletal proteins; microtuble, microfilament, and intermediate-sized-filament proteins participate in mitosis, mobility, formation and maintenance of cells, as well as in malignant transformation. Immunohistochemical stainings of those proteins are available for differential diagnosis of tumor or investigation of several pathological lesions. Monoclonal antibodies to them have been used for more specifically detect to the subject.In the present study, total 246 cases of epithelial lesions and tumors; bening epithelial lesions (82), semimalignant lesions from epithelial origin (14), basal cell carcinomas (26) and squamous cell carcinomas (124) were examined by the use of immunohistochemical stainings for anti-total keratin (TK; 41-65 kDa), monoclonal anti-keratins (KL1; 55-57 kDa, PKK1; 41-56 kDa), vimentin, clesmin and actin.In normal stratified squamous epithelium, TK and KL1 showed strong staining in granular and upper spinous cell layer, moderate in lower spinous layer and negative in basal cell layer. PKK1 was limited to basal layer cells. Normal stratified squamous epithelia expressed regular distribution to keratin proteins. Almost all papillomas and other benign epithelial lesions indicated regular distribution of keratins as in normal epithelia. In Bowen's disease, the staining of TK and KL1 displayed an irregular pattern and that of PKK1 disappeared in basal layer cells. Basal cell carcinoma showed irregular distribution of TK and, KL1 and had negative to PKK1 staining. Squamous cell carcinoma showed irregular distribution to TK and KL1 with little decreased staining, and negative PKK1. Staining of vimentin, clesmin and actin were generally negative in benign lesions and tumors, however, these proteins existed in a few cases of squamous cell carcinoma. These findings suggested that cytoskeletal proteins in malignant tumors may change in their expression.