辛基酚胁迫对雄性泥鳅抗氧化酶及卵黄蛋白原的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 辛基酚胁迫对雄性泥鳅抗氧化酶及卵黄蛋白原的影响 DOI: 10.5846/stxb201306091553 作者: 作者单位: 延安大学生命科学学院,延安大学生命科学学院,延安大学生命科学学院,延安大学生命科学学院,延安大学生命科学学院 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金项目(31240076);陕西省自然科学基础研究计划项目(2012JM3012);陕西省高水平大学建设专项资金资助项目(2012SXTS03);陕西省教育厅科学研究项目(2013JK0710);延安市科学技术研究发展计划项目(2012KS-15);延安大学博士科研启动项目(YD2007-112);陕西省生态学重点学科专项;国家大学生科技创新项目(201210719020) Incidence of antioxidant enzymes and vitellogenin induced in male loach (Misgurnus anguillicaudatus) exposed to octylphenol Author: Affiliation: College of Life Science, Yan’an University,,,, Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为研究辛基酚 (OP) 对雄性泥鳅抗氧化酶活性及血清卵黄蛋白原 (VTG) 含量的影响,将雄性泥鳅分别暴露于4 种不同质量浓度OP(0.12、0.19、0.32、0.52 mg/L)中持续7、14、21 d和28 d,采用试剂盒检测肝脏超氧化物歧化酶(SOD)与过氧化氢酶(CAT)的含量,采用碱不稳定性蛋白结合磷法检测血清 VTG 的含量。结果表明,0.12 mg/L OP 胁迫14 d,肝脏SOD 和CAT 含量均无显著变化,但是随着胁迫剂量增大和时间延长,SOD 和CAT 含量降低极其显著,在0.52 mg/L OP 胁迫28 d 时降到最低水平;泥鳅在0.12 mg/L OP 中暴露7 d 时,血清VTG 含量就有极其显著升高,且随着胁迫剂量增大和时间的延长,VTG 含量呈升高趋势。提示OP 胁迫对SOD 和CAT 活性有显著的抑制作用,并随胁迫剂量增大和时间延长而抑制加剧,造成氧化损伤;OP 胁迫可诱导VTG 合成,并随暴露剂量增大和时间延长而诱导增强,具有明显的雌激素效应,这可能与其氧化损伤有密切关系。 Abstract:Octylphenol (OP), a group of alkylphenol polyethenoxy ethers (APEs), is usually present in polluted water. OP has strong estrogenic activity, is easily concentrated and is difficult for animals to degrade; therefore, it may affect the function of the liver of Misgurnus anguillicaudatus. The antioxidant enzyme system is an important protection mechanism in the body that prevents oxidative damage during metabolism of oxygen free radicals. Although some studies of the toxicity of many phenolic contaminations have been conducted in fish and mammals, few data are currently available regarding the oxidative damage and estrogenic activity of OP in fish. To investigate the damage and activity of M. anguillicaudatus, the median lethal concentration (LC50) of OP for 24, 48, 72 and 96 hours and safe concentration (SC) were calculated by the single factor toxicity test. The LC50 for 24, 48, 72, and 96 hours were 3.26, 2.69, 2.12 and 1.82 mg/L, and the SC was 0.54 mg/L. Based on the SC values, male loaches were exposed to OP at four different concentrations (0.12, 0.19, 0.32 and 0.52 mg/L) and the activities of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) in the liver were determined at 7, 14, 21 d and 28 d. Additionally, the levels of serum vitellogenin (VTG) were examined by measuring the alkali-labile phosphorus content of the lipid-free protein fraction at 7, 14, 21 d and 28 d. The results showed that the activities of SOD did not differ significantly in response to 0.12 mg/L of OP during 14 d, but that they declined significantly with increased exposure time and concentration, with the lowest activity being observed in response to exposure to 0.52 mg/L of OP for 28 d. The CAT activities showed a similar pattern. When compared to the control group (0 mg/L), the levels of serum VTG increased significantly in response to 0.12 mg/L of OP at 7 d, and increased further with increasing concentration. As the exposure time increased, the total VTG activity in the four concentration groups also increased, with the maximum value being observed in response to exposure to 0.52 mg/L of OP for 28 d. These findings indicated that OP had high toxicity toward M. anguillicaudatus and that moderate and high concentrations of OP were able to induce oxidative stress and exert a significant negative impact on SOD and CAT activities of M. anguillicaudatus. Moreover, the inhibition intensified remarkably as the exposure concentration and time increased, resulting in the hepatic defense function of antioxidant enzymes being reduced. This, in turn, caused excess reactive oxygen species (ROS) accumulation, which can lead to oxidative damage of M. anguillicaudatus. The serum VTG could be induced in male loaches treated by OP and the induction intensified greatly with exposure, indicating that OP possesses estrogenic activity that intensified as the exposure concentration and time increased, leading to damage to the reproductive system of M. anguillicaudatus. These results indicate that the estrogenic effect of OP may be closely correlated with oxidative damage in M. anguillicaudatus. 参考文献 相似文献 引证文献