エミット法による血清中の低濃度フェニトインの測定

Abstract

Determination of lower level of serum phenytoin (PHT) was made by homogeneous enzyme immunoassay (Emit) in the following methods:(A) Wide range calibration method, (B) Extrapolated calibration method, and (C) Mixed sample method. In the method A and B, calibration curves of serum PHT levels were generated using a nonlinear least-squares regression program (EMITFIT) with a microcomputer. It was concluded that method A was most reliable of the three. In method A, the PHT recoveries in spiked serum samples in the concentrations of 0.25, 0.5, 1.0 μg/ml were 104.6, 102.6, 100.1%, and coefficients of variation were 11.4, 6.8, 2.3%, respectively.