Abstract
Angiogenesis, the induction of new capillaries and venules, is associated with tuomr growth and may promote the metastasis of tumor cells. Tumor angiogenesis in rabbits with VX 2 carcinoma of the tongue was investigated to determine relations with tumor size, metastasis, and proliferative activity. After implantation in the rabbit tongue, VX 2 carcinoma grew slowly until day 12 and then started to grow rapidly and metastasize. To estimate the proliferative activity of the tumor, the ratios of proliferating cell nuclear antigen (PCNA) were computer-generated with a CAS 200 image analysis system. The PCNA positive area was small (50.54%) on day 7 and increased significantly (73.19%) on day 12, Microvessels were immunohistologically stained with anti-CD31 antibody and counted. Microvessel density was low (29.35) on day 7 and increased significangly (70.1) on day 12. Concomitant increases in PCNA positive area and microvessel density suggest that tumor angiogenesis is related to the prolferative activity of VX 2 carcinoma of the tongue. Angiographic and vascular cast studies also suggested the presence of newly-formed microvessels in the tongue tumor. Furthermore, PCNA-positive cells in tumor nests were frequently observed near highly vascularized areas. These data suggest that tumor angiogenesis plays an important role in the progression of VX 2 carcinoma of the tongue.
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