Abstract
A new, sensitive colorimetric determination of tetracyclines is described. Tetracyclines react with diphenylborinic acid in ethanol and form yellow chelate compounds of 1:2 combination ratio. This chelate formation was utilized for colorimetry. To the aqueous solution (5cc.) of tetracycline (10-30γ), 5cc. of 1% ethanolic solution of diphenylborinic acid and 99% ethanol are added to make the whole volume 10cc. The blank test is carried out with 5cc. of distilled water, 5cc. of the reagent, and the whole volume brought to 10cc. with 99% ethanol. Optical density at maximum absorption of 415mμ is measured after one hour.Chlor- and oxy-tetracyclines can be determined in a similar manner, using the respective absorption maxima at 417 and 407mμ. The values obtained by the present method and by biological assay on aqueous solution of tetracycline allowed to stand for a long period of time were in good agreement.
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