頻回血小板輸血患者の改良ＭＰＨＡによる抗ＨＬＡ抗体スクリーニング法の応用

Abstract

As to the method of detecting anti-HLA antibody by using in the serum of patients who were frequently transfused of platelet, and by allowing it to react with lymphocyte by means of previous LCT (lymphocyte cytotoxicity test) method or AHG-LCT (antihuman immunoglobulin-LCT) method, there had been a problem that this method was difficult to be made popular as a general rutin test, because it was difficult to secure and preserve the panel lymphocytes. The authors developed a method in which HLA antigen was extracted from platelet into a physiological saline solution of 3% sucrose and it was employed as the platelet soluble antigen and fixed as a layer on the U-type Teraksaki plate, and then the screening of anti-HLA antibody was conducted by the use of this plate using the MPHA (mixed passive haemagglutination test). The authors consider that this method is useful because it has such an advantage that the platelet soluble antigen can be constantly prepared for a long period at -80°C, and further that the platelet specific antigen is also detectable, and because this method is capable of use like a commercial screening panel of erythrocyte antibody.