ラットの歯周組織創傷治癒過程に及ぼす水浸拘束ストレスの影響

Abstract

Many previous studies have investigated local factors as the causes of periodontal diseases, but there has been few research about general factors. The purpose of this study is to examine the influences of restraint and water immersion stress on wound healing in periodontal tissue and systemic conditions. The rats were divided into four groups. I a) G.E. (+) Stress (+) group=After gingivectomy, rats were stressed by being placed in a cage which was immersed in water, then sacrificed. b) G.E. (+) Stress (-) group=After gingivectomy, the rats were put back in non-immersed cages, then sacrificed. II a) G.E. (-) Stress (+) group=The rats were put under stress condition without gingivectomy, then sacrificed. b) G.E. (-) Stress (-) group=The rats were sacrificed without stress and gingivectomy. The rats of groups I a) and I b) were sacrificed at the interval of 44 hours after gingivectomy. The rats of group of II a) were sacrificed after stress, and the rats of group II b) were sacrificed without stress and gingivectomy. Histopathologically, specimens were examined by hematoxylin-eosin staining and PCNA immunohistochemical staininig. And, to demonstrate stress, views of stomach were examined. The results are as follows. Bleeding and erosion of the stomach mucous is found in all rats under stress. This confirms that the rats suffered stress. 1) There is no correlation between the stress group and the non-stress group in the length of epithelial migration from the residual epithelium of the wound site. 2) The number of cells in which the nucleus was stained by PCNA immunohistochemical staininig is significantly larger in the non-stress group than in the stress group. As mentioned above, stress may be a risk factor of periodontal disease.