Abstract
By means of laser-excited fluorescence microscopes, fluorescence intensity and its spatial distribution of hematoporphyrin dihydrochroride (Hp. 2HCl) uptaken in cultured cancer cells (TE-2) were measured quantitatively and qualitatively. It was shown that Hp. 2HCl is mostly uptaken in the individual cells for about an hour. We also found that a linear relation exists between the fluorescence intensity and the Hp. 2HCl concentration in MEM solution, and Hp. 2HCl is uptaken mainly in cytoplasma through quantitative measurements of spatial distribution of fluorescence intensity employing a SIT camera and a temporal analyzer. Moreover, observed relations of the site producing photochemical or photodynamical effects with the site of Hp.2HCl uptake in the cell are examined and discussed.
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