三次元培養真皮モデルの収縮過程に及ぼす細胞成長因子の影響

Abstract

During wound healing process, dermal fibroblasts generate a contraction force stimulating cell infiltration and collagen secretion to accelerate the wound healing process as well as decreasing the surface area of the wound. The disorder of this contraction force has risks to cause a skin disease called “keloid” by excessive cell proliferation and secretion of collagen. Keloid is sometimes accompanied with severe itchiness and pain. However, there are not any ideal treatment methods because the occurrence mechanism has been still unclear. Therefore, it is important to monitor the contraction force during wound healing process and to evaluate the effect of several growth factors working in the healing process on this force. Usually, the dermis tissue is reconstructed by embedding the fibroblasts in collagen gel and cultured in vitro. This dermis tissue model would contract during in vitro culture to simulate the wound contraction phenomena under the healing process. In this study, we developed the culturing device for dermis tissue model to enable monitoring the contraction force. This device allowed the culture of dermis tissue model by simulating the condition of CO2 incubator using the glass heater and gas controller. The effects of ascorbic acid, FGF-2 and cell concentration on contraction process were evaluated using this device. As the results, it was suggested that some growth factors were related to contraction process in three-dimensional dermis tissue model.