两个转抗真菌基因水稻根部内生真菌群落变化的分析

Abstract

本文采用真菌分离培养法和直接染色观测法分析了两个转基因根内真菌群落变化。分离培养法表明，与非转基因水稻相比，不同生长期两个转基因水稻的内生真菌分离频率和多样性与对照无显著差异。Gaeumannomyces为水稻根部内生真菌优势种群，优势度达23.23%，在转基因的两个品种和对照均可分离到，在不同的生长期也分离到。染色观测法表明，转基因水稻的内生真菌侵染率在秧苗期与对照有显著性差异，表现在它们的侵染率分别低于对照21.25%和26.56%，但在分蘖期和成熟期均无显著性差异。因此推论，水稻转该基因后，对水稻根生内真菌群落无显著影响。 Endophytic fungal community in roots of two transgenic rice (antifungal genes) was studied by isolation and staining observation. Compared with non-transgenic rice, the isolation frequency and diversity of the endophytic fungi from two transgenic rice in different growth stages exhibited no significant differences in contrast with the control. Gaeumannomyces was the dominant population of endophytic fungi in the roots of rice with the dominance of 23.23%, and could be isolated in either the two transgenic rice or the control, and any growth stages. By staining observation, the infection rates of endophytic fungi in seedlings of transgenic rice were significantly lower than the control by 21.25% and 26.56% respectively, but there were no significant differences in tillering and maturity. Therefore, it is inferred that the transgenic rice had no significant effect on fungal communities in rice roots.