Abstract
High natural agglutinin activities against sheep erythrocytes were observed in the extracts of the gill (mean titer: 141) and digestive diverticula (DD, mean titer: 128) from adult oyster, Crassostrea gigas, under hanging culture. Lower but considerable activities were found in the extracts of the mantle and adductor muscle. The natural bactericidal activity against HS 29 strain (Arthrobacter sp.) was conspicuously high in the DD extracts (mean titer: 231) and detectable also in the mantle and gill. The natural agglutinin activites of DD and gill declined sharply with progressive develop ment of the gonad and was minima just before and during the spawning, However, these activities showed a rapid rising trend after the spawning season. The agglutinin activities of DD and gill were enhanced by the injection of 0.1ml/oyster of 10% sheep erythrocyte suspension into the connective tissue surrounding DD. The bactericidal activity of DD was observed to increase after the injection of formalin-killed HS 29 strain vaccine which was given into the connective tissue surrounding DD (dose: 1.8×108 cells/g fresh soft-body wt.) or the adductor muscle (8.3×105 cells/g fresh soft-body wt.).
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