薄皮甜瓜采后病害生防菌株的安全性研究

Abstract

目的：研究对薄皮甜瓜采后病害具有防治作用的生防菌株浅黄假单胞菌的安全性，为微生物农药的开发提供安全的微生物资源。方法：将浅黄假单胞菌(Pseudomonas luteola)以3 × 109 cfu/mL的最大浓度、0.4 mL/10 g的最大体积，对小鼠二次灌胃给药，测定半数致死量和最大耐受量。结果：观察21 d，20只小鼠均状态良好，未出现任何不适现象，皮毛光亮、活动正常、摄食正常、无腹泻发生，无死亡病例。结论：该受试样品作为微生物农药使用是安全的。 Aim: To study the security of biocontrol strain Pseudomonas luteola that has better control effect on post-harvest diseases of melon, and then provide effective and safe microbial resource for the development of microbial pesticide. Methods: By second lavage in mice with maximum concentration 3 × 109 cfu/mL and 0.4 mL/10 g maximum volume, to measure the LD50 and maximum tolerated dose. Results: After having been observed for 21 days, 20 mice were in good condition and without no any discomfort; fur was light; activities and feeding were normal; no diarrhea occurred and there were no deaths. Conclusion: Pseudomonas luteola was safe for application as microbial pesticide.