Abstract

The digestive organs of carp were examined histochemically by a substrate film method, instead of the tissue homogenization procedure hitherto used in studying this subject. In the carp, amylase activity was detected in the cytoplasm of the acinous cells of the pancreas, on the luminal surfaces of the pharynx and the intestinal bulb, and on the outside of the muscularis and serosa of the intestinal bulb. The presence of amylase in the cytoplasm of the pancreatic acinous cells suggests that these are the center of amylase production in the carp, exclusive of the mucosa of the digestive tract and the hepatic cells. Amylase on the luminal surface of the digestive tract is certainly secreted from the pancreas. Amylase on the outside of the tract may derive from the pan-creatic ramification adhering to the tract.

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