Abstract
1. Gas exchange (in a phosphate buffer, pH 6.0, M/30) of intact germ-axes and slices of various parts of cotyledons isolated from soaked seeds of Vigna sesquipedalis were examined. The Qo2 on a dry weight basis and the RQ were found to be 7.8 and 1.0 for germ-axes and 0.52 and 0.81 for intact cotyledons, respectively. The Qo2 of outer part of cotyledons (1.30) was strikingly higher than that of inner part (0.44) and the RQ of the former (0.82) was lower than that of the latter (0.96). 2. Sodium fluoride and monoiodoacetate inhibited gas exchange of germ-axes strongly, but affected little that of inner part of cotyledons.3. Cyanide and azide inhibited O2 uptake of both germ-axes and inner part of cotyledons much strongly. Oxygen uptake of inner part of cotyledons was inhibited by carbon monoxide more considerably than that of germ-axes. The inhibition was completely (in the latter tissues) or almost completely (in the former) removed by light. Partial suppression of O2 uptake of these two tissues was also evoked by the addition of dieca, salicylaldoxime, α, α'-dipyridyl oro-phenanthroline.4. 2, 4-dinitrophenol stimulated O2 uptake little and CO2 output considerably of germ-axes, whereas it stimulated strongly both O2 uptake and CO2 output of inner part of cotyledons. Essentially similar results were obtained with azide. 5. Gas exchange machineries of the germ-axis and the cotyledon tissues were briefly discussed.
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