保存血液中のリンパ球活性

Abstract

It is known that viability of lymphocytes decreases gradually with storage. We studied the change of viability of lymphocytes during storage of blood under the condition of blood bank by measuring the mitotic activity.Lymphocytes were serially harvested from blood stored at 4°C in polyvinylchloride containers and the mitotic activity was assayed by the uptake of 3H-thymidine in mixed lymphocyte culture (MLC response) and in mitogen-stimulated culture with phytohemagglutinin (PHA) or concanavalin-A (Con-A).The viability determined by MLC response fell to 70, 20 and nearly zero percent of initial value after 1, 2 and 3 weeks, respectively. The response to mitogens, both PHA and Con-A, decreased earlier with storage than to allogenic leukocytes.The results indicate that mitotic activity of leukocyte is reduced to less than one-fifth initial value within 14 days and completely lost after storage of 21 days. Therefore, use of blood stored for more than 14 days is recommended for diminishing the risk of graft-versus-host disease following transfusion.