药用大黄染色体的核型分析

Abstract

对药用大黄(Rheum officinale Baill.)的染色体核型进行研究。采用常规根尖压片法分析染色体特征，并利用显微摄影技术对染色体进行分析。结果表明，药用大黄的核型公式为2n = 2x = 22 = 4M + 18m，核型类型为“1A”，核型不对称系数(As.K.%)为54.81%。染色体倍性为二倍体，不存在多倍化现象。药用大黄染色体的数量稳定，形态清晰，可为进一步深入研究奠定基础。 The karyotype of Rheum officinale was analyzed in the present study. The chromosome features were conducted by conventional root-tip squashing method and photomicrography technique. Results were as follows: The karyotype formula of R. officinale from Luanchuan in Henan was 2n = 2x = 22 = 18m + 4M; karyotype was 1A type; karyotype asymmetry index (As.K.%) was 54.81%. Chromosome ploidy was diploid, and no polyploidy was observed. The chromosome number and the morphology of R. officinale are stable and clear, which can provide the foundation for further research.