脱细胞猪小肠粘膜下层材料体内外降解过程对比研究

Abstract

通过对脱细胞猪小肠粘膜下层基质材料进行体内外降解实验，考察了SIS材料在不同降解环境下的降解行为，为其在临床应用中的有效性提供依据。体外降解实验采用I型胶原酶，蛋白酶K酶解来模拟体内降解环境；体内降解实验采用大鼠和新西兰兔分别进行皮下以及腹壁植入。结果显示SIS材料在体内修复组织需要8~12周，相当于体外I型胶原酶降解12 h (降解40.24%)，蛋白酶K降解60 min (56.33%)；材料在体内完全降解的时间为24周，而体外胶原酶和蛋白酶K完全降解分别需要96 h和120 min，同时SIS材料组织相容性好，无黏连，血肿等症状发生。结果表明SIS降解与组织修复过程相匹配，排异反应弱，可以为组织修复提供力学强度与生物学模板，表明SIS材料是一种可用于临床修复的生物材料。 Degradation behaviors of acellular porcine small intestinal submucosa (SIS) matrix under different circumstances were investigated by taking the degradation experiments in vivo and in vitro respectively. In vitro, solutions of collagenase type I and Proteinase K were used to mimic the de-gradation environment. Wistar mice and New Zealand Rabbits were used as subcutaneous and abdominal degradation model in vivo. Results showed that it took 8-12 weeks for the tissue to re-pair in vivo, while the time intervals needed to get the same degradation percentage for collagenase I and Proteinase K in vitro were 12 h and 60 min respectively. SIS was totally degraded in 24 weeks in vivo and the corresponding time for collagenase I and Proteinase K in vitro were 96 h and 120 min. Besides, SIS showed good histocompatibility and did not have symptoms of adhesion and hematomas. Results demonstrated the processes of degradation and tissue repair were matched well with each other without immunological rejection and SIS could supply the mechanical strength and biological template during tissue repair, suggesting SIS is a potential surgical biomaterial for clinical applications.