Abstract
The present study was designed to find the lowest acceptable temperature for liquid preservation of red blood cells. We stored red cells at 5°C, 2.5°C, 0°C, -2°C and -5°C for 6 weeks in citrate-phosphate-dextrose (CPD) solution. Supernatant hemoglobin concentration was maintained at the low level at 0°C throughout the storage, but showed a significant increase at a temperature below -2°C. A temperature of 0°C was the most effective in the maintenance of the adenosine triphosphate (ATP) level, morphology score, and erythrocyte deformability, indicating that the red cells stored at 0°C were the most viable. The 2, 3-diphosphoglycerate (2, 3-DPG) level during the storage increased in the order of 5°C<0°C<-2°C, -5°C. These data suggest that the temperature for red cell preservation can be lowered to 0°C. Judging from the ATP level and morphology score, the storage period of red blood cells could be extended up to about 5 weeks even in CPD solution if the red cells are stored at 0°C.
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