Abstract
For a long time, the resolution of optical microscopy has been limited by diffraction. In recent years, many methods have been developed to break the diffraction barrier. Among them, stimulated emission depletion (STED) microscopy becomes very popular for being able to provide high speed and high resolution imaging. The pulsed STED can provide higher resolution but its construction is rather complex. STED with continuous wave can be built easier but its resolution is deteriorated. Applying time gating in the STED with continuous wave (gSTED) can further improve the resolution. In this paper, we discussed the principle and the application of time-gating in STED.
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