Abstract

Fibrin glue is used widely in Japan as tissue sealant, adhessive and hemostatic agent. But it is prepared commercially from pooled human blood, so there is some risk of transmission of hepatitis B, acqired immune deficiency disease, and other blood-transmission disease. We described a cryoprecipitation technique for preparation of fibrin glue from patient's own blood. This technique enables the glue to be made in large quantities with no greater risk of disease transmission. We report on our method preparing fibrinogen from single-donor fresh-frozen plasma (FFP). We have found that the cryoprecipitation glue is useful in reducing post-operative bleeding in hip surgery and that it seems to affect osteogenesis.

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