たんぱく質工学の展望

Abstract

The level theory of protein structure ; module and domain theory in connection with the molecular evolution will become a useful theory for designing new functional proteins. Chemical and enzymatic modification of proteins is a useful technique. Site directed mutagenesis is extensively used for the conversion of amino acid residues of enzymes. Some of the examples gave successful results for the increase of enzymatic activity or heat stability. Instead of changing amino acids one by one, very recently a hybrid enzyme and multi enzyme peptides are designed and produced. Many proteins produced in E. coli are often inactive because of the failed conformation. One of the resolution of this problem is to secrete the products into culture media. Secretary production of protein by yeast is emphasized. Recent production of proteins by yeast gave a good prospect, but many things remain vague.