Abstract
Change of surface charge of the tanned sheep erythrocytes added with supernatants of human lymphocytes stimulated with antigens, mitogens or immunopotentiators was investigated. In supernatants of the sensitized lymphocytes stimulated with PPD, percentage slowing of electrophoretic mobility of sheep erythrocytes were increased. In normal lymphocytes cultured with PHA and Con A, there were high percentage slowings in electrophoretic mobility test, and in immunopotentiators such as OK-432, PSK, LVS, methyl B12, poly-A:U, poly-I:C and NaIO4, percentage slowings were not significantly changed.Percentage slowing was reduced in RA lymphocytes stimulated with PHA, but not changed in SLE lymphocytes. In normal lymphocytes incubated with Lentinan, percentage slowing value was similar to the result obtained by Con A, but it was low in lymphocytes from the patients with collagen diseases and malignancies.In SLE lymphocytes added with DNA from calf thymus and RA lymphocytes added with human IgG, electrophoretic mobility of sheep erythrocytes delayed.
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