Abstract
The genus of Chrysobothris Eschscholtz, 1829 is one of the most diverse and widespread genera of the family Buprestidae of some 700 described species distributed throughout the world. In Iraq, particularly in the Kurdistan region, about 4 species had been recorded so far, many of these species are sympatric, share larval host plants, and are difficult to reliably separate morphologically. The current study investigates species limits and relationships among the recognized species occurring within the Erbil Province; mitochondrial cytochrome C oxidase (COX I) molecular analysis confirmed the monophyly of two Chrysobothris species, Ch. affinis (Fabricius, 1794) and Ch. chrysostigma (Linnaeus, 1758). Implications of the resultant larval morphology and molecular techniques are discussed. Diagnostic characteristics that are depended to identifying the species within Chrysobothris in larval stage were illustrated and then compared with the molecular data.
Highlights
Chrysobothris Eschscholtz, 1829 is a common genus widely distributed throughout the world (MacRae and Basham, 2013)
The larvae develop in the sapwood of the stems of the apricot Prunus armeniaca L., 1753, peach Prunus persica L., 1801, plum Prunus domestica L., 1753, pear Pyrus communis L., 1753 and pomegranate Punica granatum L., 1753
This genus is one of the most diverse and widespread genera of the family Buprestidae of some 700 described species distributed throughout the world (Hawkeswood, Morphology and molecular identification 1995; Bellamy, 2002)
Summary
Chrysobothris Eschscholtz, 1829 is a common genus widely distributed throughout the world (MacRae and Basham, 2013). The larvae develop in the sapwood of the stems of the apricot Prunus armeniaca L., 1753, peach Prunus persica L., 1801, plum Prunus domestica L., 1753, pear Pyrus communis L., 1753 and pomegranate Punica granatum L., 1753 This genus is one of the most diverse and widespread genera of the family Buprestidae of some 700 described species distributed throughout the world It is necessary for proper identification to provide a detailed larval description which is based on the optic and on molecular technique These species were found to be common and destructive pests in Kurdistan orchards. Molecular sequencing techniques provide a precise other approach to suppose evolutionary relationships among closely related species (Rubinoff et al, 2006; Hansen et al, 2015)
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