Abstract
The amounts of DNA and proteins contaminated in fermented ethanol rectified for industrial use were investigated. Ethanol samples were evaporated to dryness, then dissolved to TE buffer or ultra-pure water. DNA was analyzed by the UV method, the Pico Green method and the quantitative PCR analysis. Protein was measured by the Bradford method and the Micro BCA method. Detection limits of each method were determined. It is confirmed that any DNA and protein were not detected in industrial ethanol products. Distillation tests adding λDNA and BSA were performed. There is no DNA and protein in distillates come from the distillation tests. Fermentation and distillation tests using GM corn were carried out. The amount of detectable DNA was gradually decreased during the fermentation and distillation processes. DNA would be degraded and eliminated in the processes. There were not any DNA and proteins detected in the distillate produced from GM corns.
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