微小電気刺激が骨再生の初期過程に及ぼす影響

Abstract

The study investigated early stage of regeneration of bone defect by an electrical stimulation in Rats. Electrical current source produced by a sheet of poling VDF/Tr FE film was used as irregular current and membrane.The subject were Wistar-strain male rats with an average age of 13 to 15 weeks, which were then randomaized into 3 test groups. Under general anaesthesia, a hole (2 mm) was drill in femoral diaphysis of each rat in all groups. In the contral group, the hole was uncovered. In Group A, poling VDF/Tr FE film was covered. In Group B, the hole was covered by poling VDF/Tr FE film which generated a maximum average voltage of +1, 531--1, 612 m V when the rat moved freely. Histopathological observations were made by light and electron microscopy.The results were as follows.1. By electrical stimulation of the film, bone regeneration was facilitated to increase the number of mesenchymal cells in the endosteal site, and induced osteoblastic differentiation after three days.2. By electrical stimulation of the film, new vascula formation had been effected to create a suitable enviroment in which bone regeneration could be facilitated after five and seven days.3. The film could facilitated bone remodeling by osteoclast after seven days and matured bone tissue at the early stage.4. The materials and electrical stimulation of the film did not obstruct dysplastic osteoblast and facilitated bone regeneration at early stage.