Abstract
It was found that the addition of nonionic surfactants, having polyoxyethylenic groups, could accelerate the rate of crystallization of hen egg white lysozyme (3, 2, 1, 17) in acidic pH, to widen the conditions of lysozyme crystallization. Crystals of lysozyme hydrochloride were obtained directly from egg white with this effect. Four types of nonionic surfactants tested, were all effective, and in the same type of the surfactant, the more hydrophilic, the more effective. Although the effect increased in proportion to the concentration of the surfactant added, the effect reached a plateau at above the definite concentration of the surfactant. A small amount of the surfactant was detected in crystals of lysozyme obtained in the presence of the surfactant.
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