レクチンの認識機能を有するハイブリッド型リポソームの調製

Abstract

Hybrid liposomes that have a targeting effect were prepared by introducing a sugar type surfactant. The hybrid liposomes consist of dimyristoylphosphatidylcholine, polyoxyethylene glycol surfactant, and a long-chain alkyl sugar ester. The targeting effect was evaluated on the basis of the binding behavior between the hybrid liposomes and a lectine. When the hybrid liposomes were prepared with a glucose type of surfactant, aggregation was observed by the addition of concanavalin A (ConA) to the liposome solution. The result indicates that bridging should occur between the sugar moiety on the surface of liposomes and the recognition site of lectine. The aggregation behavior was also confirmed by the AFM photograph. The interaction between the hybrid liposomes and lectine was found to be reversible because the aggregates disappeared by the addition of excess glucose. Hybrid liposomes containing a galactose type of surfactant exhibited a high affinity toward the peanut lectine (PNA). The alkyl-chain length of sugar surfactants also affected the aggregation behavior. It was suggested that longer alkyl chain surfactants show a higher recognition effect.