Abstract
We have performed in vitro experiments of laser irradiation on osteoblastic cells to investigate the effect on cell differentiation and calcification. Clonal osteoblastic cells (MC3T3-E1) were exposed to Helium-Neon laser beam at various energy densities. 45Ca accumulation was enhanced by laser irradiation at 1.0 J/cm2, with four sessions of irradiation resulting in a 46%increase as compared with the control. In contrast, no significant increase in alkaline phosphatase activity was produced by laser irradiation. Electron microscopic observation revealed a tendency of enlargement of the Golgi apparatus in the laser-treated cells. In clinical use, laser therapy was given to eight patients with fractures, and we obtained good results. These findings suggest that laser irradiation photoactivates osteoblastic cells, accelerates osteoblastic calcification as well as cell proliferation, and therefore, could promote bone regeneration. Laser therapy might be of therapeutic value as a promising modality for fractures.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: THE JOURNAL OF JAPAN SOCIETY FOR LASER SURGERY AND MEDICINE
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
