Abstract
目的：研究太平洋折翅蠊(Diploptera punctata)中一种柠檬酸转运载体(Citrate carrier, CiC)在保幼激素(juvenile hormone, JH)合成通路中的功能，探讨该CiC与JH合成的关系，为针对保幼激素途径进行的新型害虫防治提供理论依据。方法：以太平洋折翅蠊为研究对象，利用荧光定量PCR技术对CiC在不同器官及咽侧体不同发育时期的表达量进行分析。结果：柠檬酸转运载体在合成保幼激素的主要场所咽侧体(Corpora allata, CA)中表达量最高，其次是脂肪体(Fat body, Fb)。在雌性蟑螂交配后0~7天内，咽侧体中CiC表达量与JH释放量成正相关。利用RNA干扰(RNA interference, RNAi)技术对JH合成关键酶3-羟基- 3-甲基戊二酸单酰辅酶A还原酶和保幼激素酸甲基转移酶基因进行干扰后，CiC表达量明显下调。结论：CiC与JH合成通路密切相关，其可能是通过介导乙酰辅酶A由线粒体到细胞质的跨膜运输，影响JH的从头合成。 Purpose: To determine the function of Citrate carrier (CiC) in the biosynthesis of Juvenile hormone in cockroach, D. punctata, and build a basic theory for pest control. Methods: The expression profile and tissue distribution of CiC were determined by qRT-PCR. Furthermore, the relationship between JH biosynthesis and CiC were investigated by knocking down the essential genes in JH biosynthesis pathway using RNAi. Results: CiC has the highest expression level in CA, which is the biosynthetic site of JH, and followed by Fb. CiC expression pattern in CA corresponds with JH biosynthesis in the CA of 0 - 7d after mated female. There is a down regulation in CiC expression after knocking down the 3-hydroxy-3-methylglutaryl coenzyme-A reductase and Juvenile hormone acid methyl transferase, two key genes that play essential roles in JH biosynthesis. Conclusion: Our result suggested CiC was involved in JH biosynthesis pathway, probably by mediating the transmembrane transport of Acetyl-CoA. Further investigate was needed to determine the accurate function of CiC.
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