石漠化生境对三叶木通生长及生理特性的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 石漠化生境对三叶木通生长及生理特性的影响 DOI: 10.5846/stxb202102250522 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家重点研发计划项目（2016YFC0502604）；贵州省科技计划重大专项课题（黔科合平台人才[2017]5411-06，黔科合平台人才[2017]5788）；贵州省高层次创新型人才培养计划项目（黔科合人才[2015]4031） Effects of rocky desertification habitat on growth and physiological characteristics of Akebia trifoliata Author: Affiliation: Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:石漠化生境土层浅薄、干旱贫瘠，许多植物难以生存。三叶木通作为石漠化生境适生物种之一，探究其对石漠化生境的应答机制对综合治理石漠化生境具有一定的理论和实际意义。在贵州省贵阳市花溪区和安顺市关岭县两地分别选取石漠化生境和普通生境（对照）种植三叶木通，并进行为期一年的观察，探究石漠化生境对三叶木通根、茎、叶等不同器官的生长和生理特性的影响，并利用主成分分析出三叶木通在石漠化生境的主要响应指标。结果显示，与对照相比，石漠化生境下的三叶木通表现出如下特征：（1）植株较矮、茎较细、叶片数较少等生物量的减少，使其适应石漠化生境；（2）叶片叶绿素a/b （Chla/b）和相对含水量（RWC）呈现降低趋势，从而使叶片净光合速率（Pn）、蒸腾速率（Tr）、气孔导度（Gs）和水分利用率（WUE）均呈下降趋势，胞间CO2浓度（Ci）呈升高趋势，利于适应石漠化生境；（3）丙二醛（MDA）含量和叶片相对电导率（RC）呈现升高趋势，说明石漠化生境对三叶木通细胞膜系统造成损伤（氧化损伤和渗透损伤），而通过其根中过氧化氢酶（CAT）、茎中过氧化物酶（POD）和叶片中超氧化物歧化酶（SOD）活性升高来防御氧化造成的伤害；通过增加可溶性蛋白（SP）、脯氨酸（Pro）等渗透性调节物质含量，防御渗透压造成的伤害；（4）主成分分析表明，MDA、POD和SP在三叶木通对石漠化应答中起着主要作用。研究揭示了三叶木通响应石漠化生境的生长及生理调节机制，为三叶木通用于石漠化综合治理提供理论基础的同时，也为进一步阐明三叶木通抵抗石漠化逆境的分子机理、提高三叶木通抗逆性提供参考依据。 Abstract:The rocky desertification habitat has a thin soil layer and is arid and barren. Many plants are sometimes difficult to survive. Akebia trifoliata is one of the species that can grow in rocky desertification habitats. Exploring its response mechanism to rocky desertification habitats has certain theoretical and practical significance for comprehensive management of rocky desertification habitats. In Huaxi District of Guiyang City and Guanling County of Anshun City, Guizhou Province, rocky desertification habitat and the common habitat (control) were selected to plant Akebia trifoliata and observed for one year. We explore the impact of rocky desertification habitat on the growth and physiological characteristics of different organs such as the roots, stems and leaves of Akebia trifoliata, and use principal component analysis to the main response indicators of Akebia trifoliata in the rocky desertification habitat. The results show that the Akebia trifoliata in the rocky desertification habitat exhibits the following characteristics than the common habitat (control):(1) the reduced biomass, such as shorter plants, thinner stems and fewer leaves, so as to adapt to the rocky desertification habitat; (2) leaf chlorophyll a/b (Chla/b) and relative water content (RWC) indicated a decreasing trend, so that the leaf net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and water use efficiency (WUE) presented a downward trend, and the intercellular CO2 concentration (Ci) had a rising trend, and then conducive to adapted to the rocky desertification habitat; (3) the content of malondialdehyde (MDA) and relative electrical conductivity (RC) of leaves showed an increasing trend, indicating that the rocky desertification habitat caused damage (oxidative damage and osmotic damage) to the cell membrane system of Akebia trifoliata. It protects against damage caused by oxidation by increasing the activities of catalase (CAT) in roots, peroxidase (POD) in stems and superoxide dismutase (SOD) in leaves. It protects against damage caused by osmotic pressure by increasing the content of osmotic adjustment substances such as soluble protein (SP) and proline (Pro); (4) principal component analysis showed that MDA, POD and SP play a major role in the response of Akebia trifoliata to rocky desertification. This study reveals the growth and physiological regulation mechanism of Akebia trifoliata in response to rocky desertification habitats, provides the theoretical basis for Akebia trifoliata's comprehensive management of rocky desertification habitats. Meantime, this study could further clarify the molecular mechanism of Akebia trifoliata resisting rocky desertification habitats, and provide a reference basis for improving its resistance to adversity. 参考文献 相似文献 引证文献