外源激素对桂北地区原生国兰组培快繁的效应探究

Abstract

利用L9(34)正交试验方法，研究IBA、6-BA两种外源激素和活性炭(AC)对桂北地区原生国兰组培快繁的效应。原球茎诱导形成阶段适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L；原球茎增殖期适宜培养基为MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L；原球茎分化期适宜培养基为MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L；壮苗及生根阶段适宜培养基为1/2MS + IBA 0.4 mg/L + AC 1.0 g/L。 The effect of IBA and 6-BA two kinds of exogenous hormones and active carbon (AC) on rapid propagation of northern Guangxi native orchid tissue culture was studied by L9 (34) orthogonal test. At the induction stage of protocorms formation, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.6 mg/L + AC 4.0 mg/L; at the proliferative stage of protocorms, the suitable medium was MS + 6-BA 1.0 mg/L + IBA 0.4 mg/L + AC 4.0 g/L; in the differentiation stage of protocorms, the suitable medium was MS + 6-BA 0.4 mg/L + IBA 0.6 mg/L + AC 0.5 g/L; in the seedling and rooting stage, the suitable medium was 1/2MS + IBA 0.4 mg/L + AC 1.0 g/L.