保存血液の物理的および化学的性質の変化について

Abstract

It is well known that physical and chemical changes occur during storage of preserved blood. Those changes, , however, depend on storage conditions. For example, there are differences depending on whether bloom is stored in glass bottles or in plastic bags. One of the main reasons for differences under these conditions seems to be oxygenation effect in the plastic bags. Preserved blood stored in plastic bags takes up oxygen through the bags. We have studied this oxygenation effect on preserved blood before and after airation in glass bottles and in plastic bags.The changes in the following properties were measured:(1) Osmotic fragility of red blood cells.(2) Hemoglobin content in plasma.(3) Viscosity of whole blood.(4) pH in plasma.(5) ATP and 2, 3-DPG contents in red blood cells.There are no significant changes in the properties from (1) to (4) after 42 days in blood stored in plastic bags, while big changes have been seen in blood stored in glass bottles after 14 days.ATP and 2, 3-DPG have been consumed in both cases, but consumption was much higher in blood stored in glass bottles. Blood stored in glass bottles after airation has shown a pattern close to that of stored in plastic bags.In conclusion, plastic bags cause less changes in the properties mentioned above. It is clear that oxygen strongly affects preserved blood as expected.