Abstract
Biotechnology of cyanobacteria takes much attention because of their ability to utilize light energy and assimilate carbon dioxide. Synechocystis species are unicellular, non-nitrogen fixing cyanobacteria, which are widely used for both basic and applied sciences. Here I introduce our study discovering new way to increase the productivity of hydrogen from Synechocystis cells. SigE, an RNA polymerase sigma factor, is a positive regulator of sugar catabolism and bioplastic production. The sigE overexpression also increases the hydrogen production during dark anaerobic conditions. Phenotypic analysis also reveals that the sigE overexpression alters the photosynthetic activity and cell sizes, indicating the close interaction among metabolism, photosynthesis, cell size maintenance, and bioproduction. These results demonstrate the novel method promoting hydrogen productivity using transcriptional regulator of cyanobacteria.
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