卵胞嚢腫のホルスタイン種経産牛における過剰排卵処理成績

Abstract

The efficacy of superovulatory treatment administered to Holstein cows (n=9) with follicular cysts was evaluated. On the day when the cows were diagnosed as having follicular cysts, all follicles ≥10mm in diameter, including cystic follicles, were aspirated transvaginally. Immediately afterwards (Day 0), an intravaginal progesterone-releasing device (controled internal drug release type B: CIDR-B) was inserted. From Day 4 till the morning of Day 7, cows were super-stimulated with a total of 37 mg FSH. On Day 7, PGF2α analog 500μg was injected, and the CIDR-B was removed. On Day 8, the cows were inseminated; and embryos were collected non-surgically on Day 15, producing 2.8 transferable embryos. For control purposes, conventional superovulatory treatments were administered to cows (n=7) with normal estrus cycles. From these animals, 4.7 embryos were collected. No significant differences were observed between the two groups. These results indicated the possibility of collecting embryos from cows with follicular cysts by using superovulalory treatment and of producing results similar to those obtained from normal cows submitted to conventional superovulatory treatment.