Abstract
Mature embryos were excised from the seeds of Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endangered species only inhabiting the south west islands of Japan. They were cultured in vitro under different tissue culture conditions. Adventitious buds were induced on the surface of the embryo on medium containing BAP and they grew to shoots after subculturing to medium containing activated charcoal or a low concentration of thidiazuron. From the elongated shoots, root primordia and roots were induced in medium containing IBA as an auxine. We found that a low concentration of zeatin or BAP added to the medium was beneficial for plant regeneration of mature embryos of this species. There were many abnormal chlorophyll germinants from seeds collected in an isolated tree.
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