Abstract
Separatory determination of mixed hormones in tablets was attempted with tablets containing ethynylestradiol and ethysterone or methyltestosterone. It is possible to determine the content of ethysterone and methyltestosterone from the ultraviolet absorption spectrum at 241mμ, without the effect of ethynylestradiol. The content of ethynylestradiol can be determined by column chromatography through alumina, removing ethysterone or methyltestosterone by elution of the column with 2% ethanolic benzene, eluting out ethynylestradiol with 30% ethanolic benzene, evaporating this eluted solution, and coloring the residue by the hydroquinone method.
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