カサゴに対する摂餌促進物質の同定

Abstract

Feeding stimulants for the marbled rockfish Sebasticus marmoratus were identfied by the omission test uding the synthetic extract of krill Euphausia pacifica. The fish were fed on a purifiedcasein diet supplemented with constituents of the extract for 6 days. The feeding stimulant activity was evaluated by the daily feeding rate. The nucleotide fraction of the extract was the most effective, followed by the amino acid fraction which showed a littel activity. Inosine had the highest activity among all constituents of the extract. With increasing supplementary level to the diet, amino acids such as L-alanine, L-methionine, L-serine and L-proline and nucleotides such as adenosine-5'-diphosphate, adenosine-5'-monophosphate and inosine-5'-monophosphate became positive. When inosine together with, L-alanine, L-methionine, L-serine and L-proline was supplemented to the diet, its activity for the fish was remarkably improved, becoming to the synthetic extract.