Abstract
It is well known that tobacco smoke causes the health risks not only for smokers but for non-smoker as well. We thus evaluated the urinary cotinine, content, which is a major metabolite of nicotine, by high-performance liquid chromatography (HPLC). Such a determination is considered to provide a useful objective assessment of exposre to tobacco smoke. Using the Previously described method, non-smokers in a typical company were thus found to appareutly demonstrate cotinine concentrations due to passive smoking, Furthermore we also determined the urinary cotinine in an asthma patient who could not stop smoking. The results indicated that the measurement of the urinary cotinine level by HPLC could be effective as a strong motivating factor to stop smoking and also to form a system of nosmoking support groups. In the future, such continuous study will enable us to approximate the effect of passive-smoking on a disease based on the measurement of the urinary cotinine level.
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