カツオおよびビンナガの濃縮エキス中のアルセノベタインの単離と同定

Abstract

The water-soluble arsenic compound was isolated from a concentrated extract of skipjack tuna (so-called “katsuo-senji” in Japanese) which was prepared from cooker drain of “katsuo-fushi” preparation, and that of albacore prepared from steaming drain of tuna canning. The specimens were extracted with a chloroform-methanol mixture, and the arsenic compounds were isolated from the methanol phases by successive chromatographies on active carbon, Dowex 50W-×8 (H+form), Dowex 1-×8 (OH-form), Sephadex G-25F, Dowex 50W-×2 (pyridinium form), and finally preparative thin layer of cellulose. Both isolated compounds were identified to be arseno-betaine by comparison of TLC behavior, IR and FAB mass spectra with those of synthetic arseno-betaine.