Abstract
Copper deposition of periportal hepatocytes indicates chronic cholestasis and it is regarded as a very important histological finding to diagnose primary biliary cirrhosis (PBC) and define its stage. Orcein stain makes copper-binding protein visible clearly and sensitively. However, there are variations in orcein staining results every institutions. In order to generalize and standardize staining method, we surveyed the methods of orcein stain at 11 institutions including our laboratory. Consequently, there were differences in staining result according to the kinds of orcein reagents and reducers. As for staining of copper-binding protein, combination of orcein reagent manufactured by Merck or Tokyo Chemical Industry and 1.5 to 3%oxalic acid as reducer is recommended.
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