Abstract
Changes in molecular species of choline and ethanolamine glycerophospholipids of rat uteri during estrus cycle and pre- and post-administration of estradiol to oophorectomized rat were studied. The change in phospholipid composition was negligible, but the molecular species of these phospholipids greatly changed. Analyses of t-BDMS derivatives of diacyl and ether types of these glyceropholipids were carried out by gas chromatography-mass spectrometry using selected ion retrieval technique.(1) The major m olecular species of choline glycerophospholipids (PC) were as follows: in diacyl type, PC32:0 (mainly C16:0/C16:0), PC34:0 (mainly C16:0/C18:0), PC34:1 (mainly C16:0/C18:1), PC34:2 (mainly C16:0 /C18:2), PC36:2(mainly C18:0/C18:2), PC36:3(mainlyC18:0/C18:2), PC36:4(mainly C16:0/C20:4) and PC38:4 (mainly C18:0/C20:4), whe reas in ether type, PC32:0 (mainly Et16:0/C16:0), PC34:0 (mainly Et16:0/C18:0), PC34:1 (mainly Et16:0/C18:1), PC34:2 (mainly Et16:0/C18:2), PC36:1(mainly Et18:0/C18:1), PC36:4(mainly Et16:0/C20:4) and PC38:4(mainly Et18:0/C20:4). Among these molecular species, in both diacyl and ether types, PC34:1, PC34:2 and PC36:2 increased but PC32:0PC36:4and PC38:4 decreased at proestrus phase and by the administration of estradiol.
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