蛍光分析を利用した動脈硬化病変の診断

Abstract

To assess whether laser-induced fluorescence (LIF) spectra could discriminate atherosclerotic plaque from normal artery, two kinds of spectral analysis were studied. 1) Spectral analysis based on LIF from arterial samples after treatment of exogenous fluorescence probe, chlortetracycline hydrochloride (CTC), 2) spectral analysis based on autofluorescence from non-treated arterial samples.The CTC-treated samples were irradiated by 308nm high power excimer laser light, and the non-treated samples by 325nm low power laser light.A spectrum from CTC-treated normal artery had a broad emission band between 350-700nm and was characterized 2 fluorescence intensity peaks at 390 and 430nm. A new characteristic fluorescence intensity peak at 540nm for CTC-treated atheromatous tissue was observed. This peak was fluorescence emission band from CTC, which fluoresced only in the form of fluorescence chelate after binding to calcium ion and this peak was enhanced in the presence of collagen.Autofluorescence spectra from normal artery was characterized by fluorescence intensity peak at 450nm, that from smooth plaque by that at 390nm and that from ulcerated plaque by low intensity and broad fluorescence emission band.Both spectral analysis could differentiate atherosclerotic plaque from normal, but autofluorescence spectral analysis seems to reflect the pathological conditions of plaque.