N-3 PUFA Supplementation Alters Retinal Very-Long-Chain-PUFA Levels and Ratios in Diabetic Animal Models.

Abstract

Long-chain (LC)-PUFAs act as precursors for the special class of retinal lipids known as very-long-chain (VLC)-PUFAs and the effect of diabetes on retinal VLC-PUFA levels is unexplored. In order to understand the supplemental effect of omega-3 (n-3) LC-PUFAs on decreasing levels of VLC-PUFAs due to diabetes, Nile rats, which develop diabetes spontaneously, and Akita mouse, a genetic diabetes model, are chosen. Human retinal punches from donors are collected from an eye bank; lipids are extracted and analyzed to study the alterations in VLC-PUFAs and their omega-3/omega-6 (n-3/n-6) ratios. Nile rats are fed a high-fat diet to induce hyperglycemia, and then an n-3 PUFA-rich diet is fed to the experimental group for 2 months. Diabetic male Akita mice and WT mice are fed with 5% fish-oil mixed in with their chow for 2 months to observe the effect of n-3 PUFAs. Results indicate that VLC-PUFA levels are lower in human diabetic and retinopathic retinal punches compared to age-matched controls. With supplementation of n-3 PUFAs, there is a significant increase in n-3/n-6 VLC-PUFA ratios in both animal models compared to diabetic controls. Dietary supplementation with n-3 LC-PUFAs helps to prevent progression of diabetes and associated retinopathy.