Abstract
A high performance liquid chromatography method is presented for the determination of valproic acid levels in human plasma. The method was based on pre-column derivatization using N-(1-naphthyl)ethylenediamine as a new labeling agent. The calibration curve was linear in the investigated concentration range between 0.1 and 100 μg mL−1 and showed good accuracy and reproducibility. The assay provided a limit of quantification of 0.1 μg mL−1 for valproic acid and a limit of detection of 10 ng mL−1, respectively. The presented method was successfully applied to the determination of valproic acid levels in plasma after oral administration of 600 or 800 mg of sodium valproate.
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