N‐(β‐Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay

Abstract

AbstractA new chemiluminescence label N‐(β‐carboxpropionyl)luminol (CPL) was used to label sheep anti‐human IgG (SaHIgG). The labeled antibody waa stable and could be detected at least dawn to 10−17 ∼10−16 mol. The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG. There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling.