Abstract
Pepsin, pronase, and trypsin were compared for their ability to digest the tissue residues that constitute the overwhelming mass of plankton centrifuge harvests. Trypsin was preferred; a treatment with 0.25% for 30 min at pH 7.2–7.5 released an average of 22% additional spores and resulted in an average 11-fold increase in spore concentration. The procedure significantly enhances the sensitivity of spore detection by the plankton centrifuge method.Key words: Myxosoma cerebralis, myxosporidans, spore detection
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