Myxobolus liuyangensis sp. n. (Myxosporea: Myxobolidae) from the gills of exotic mrigal carp Cirrhinus mrigala (Actinopterygii: Cyprinidae) in China.

Abstract

A new species, Myxobolus liuyangensis sp. n., was found in the gills of the exotic mrigal carp Cirrhinus mrigala during a survey of the fauna of exotic fish myxospore in China. Plasmodia were elongated pyriform, measuring 0.42 mm long and 0.15 mm wide. The mature spores were elongated pyriform in the frontal view, tapered forward, rounded posterior end, and fusiform in the sutural view, measuring 17.3 ±0.5 (16.5-18.3) μm long, 6.2 ±0.3 (5.2-6.8) μm wide, and 4.8 ±0.2 (4.4-5.1) μm thick. The two equal polar capsules of elongated pyriform in shape measured 11.3 ±0.5 (10.6-12.3) μm long and 2.5 ±0.3 (2.0-3.1) μm wide, occupying more than half the capacity of the spores. The polar filaments were coiled with fifteen to sixteen turns. No mucous envelope and caudal appendages were found. The consensus SSU rDNA gene sequence obtained here for M. liuyangensis sp. n. did not match any sequences available in GenBank, but was most closely related to M. catlae that infects the gills of C. cirrhosis (MT003664, 97.99% similarity). Phylogenetic analysis indicated that the C. mrigala-infecting Myxobolus species were not clustered together, but dispersed in different clades. The present species clustered with M. catlae and M. orissae within the clade I of elongated pyriform spore shapes, revealing spore shapes may play an important role during the evolution of Myxobolus species. This is the second myxosporean infection report in the exotic mrigal carp C. mrigala.